It is generally accepted that baseline noise in liquid chromatograph detectors is a more common failure than lamp failure. Baseline noise is greater within 30 minutes after the deuterium lamp is lit, so observe baseline conditions at least 30 minutes after each use. In addition, the lamp after a long period of use will have an increase in noise due to reduced energy.
The following methods can be used to confirm the direction of the source of liquid chromatograph noise:
1 Retest the baseline under standard reference conditions;
2 Replace suspect detectors (replace rules) with detectors that are believed to be problem-free to determine whether the detector is a problem;
3 Change lamp. (Although the price of the lamp is more expensive, it is easier to change the lamp than to use other methods to find a fault.) If there is always a long noise tip in the chromatogram, you can stop the pump or set the flow rate to 0mL/min to compare the noise source. If the bubble causes sharp noise, it will not appear after stopping the pump. If it is a problem of the lamp, stop the pump after exist. It is worth noting that the noise of the lamp itself increases after the pump is turned on.