Ultrasonic cell disruption instrument, also called ultrasonic cell pulverizer, is a multi-functional, multi-purpose instrument that uses cavitation to generate cavitation effects in liquids and ultrasonically treat substances. It can be used for the crushing of various animal and plant cells and virus cells. At the same time, the ultrasonic cell disrupter can be used for emulsification, separation, homogenization, extraction, defoaming, washing and accelerating chemical reactions.
principle
The principle of ultrasonic cell disruption is not too mysterious and complicated. Simply put, the electric energy is converted into sound energy through the transducer. This energy is turned into a dense small bubble through the liquid medium. These small bubbles burst rapidly and produce the same energy as a small bomb, thus breaking the cell. The role of substances.
Ultrasonic is an elastic mechanical wave in a matter medium, which is a form of wave, so it can be used to detect physiological and pathological information of the human body, and to diagnose ultrasound. At the same time, it is a form of energy. When a certain dose of ultrasound is transmitted through the body, the interaction between them can cause changes in the function and structure of the organism, that is, the ultrasonic biological effect. The effects of ultrasound on cells are mainly thermal effects, cavitation effects and mechanical effects. The thermal effect is that when the ultrasound propagates through the medium, the friction hinders the molecular vibration caused by the ultrasound, converting part of the energy into local high heat (42-43 ° C), because the critical lethal temperature of normal tissue is 45.7 ° C, and the tumor tissue ratio Normal tissue sensitivity is high, so at this temperature, the metabolism of tumor cells is impaired, DNA, RNA, and protein synthesis are affected, thereby killing cancer cells and normal tissues are not affected. The cavitation effect is that under the irradiation of ultrasound, vacuoles are formed in the living body, and mechanical shear pressure and turbulence are generated along with the vibration of the vacuole and its violent explosion, which causes the tumor to hemorrhage and the tissue to collapse and cause necrosis. In addition, when the cavitation bubble bursts, it generates instantaneous high temperature (about 5000 ° C) and high pressure (up to 500 × 104 Pa), which can thermally dissociate the water vapor to produce .OH radicals and .H atoms, which are composed of .OH radicals and .H. Atomic induced redox reactions can lead to polymer degradation, enzyme inactivation, lipid peroxidation, and cell killing. The mechanical effect is the primary effect of ultrasound. During the propagation process, the medium particles alternately compress and stretch to form a pressure change, causing damage to the cell structure. The intensity of killing is closely related to the frequency and intensity of ultrasound.
Structural features
â—Ultrasonic probe is made of imported titanium alloy
â—Energy efficient transducer
â—Amplitude is automatically adjusted to maintain the same amplitude under different load conditions
â—Set the ultrasonic intermittent time
â—Microcomputer control, continuous adjustment of ultrasonic power
â— Integrated temperature control sample temperature
â—The soundproof box is made of special soundproof material
The main purpose
Ultrasonic cell disruption instrument has the functions of crushing tissues, bacteria, viruses, spores and other cell structures, homogenization, emulsification, mixing, degassing, disintegration and dispersion, leaching and extraction, accelerating reactions, etc., so it is widely used in biology, medicine, Laboratory research and enterprise production in chemical, pharmaceutical, food, cosmetics, environmental protection, etc.
Purchase attention
Ultrasonic generator
The signal generator generates a signal of a specific frequency, which is the frequency of the transducer. The ultrasonic frequencies generally used in the ultrasonic device are 20KHz, 25KHz, 28KHz, 33KHz, 40KHz, 60KHz.
Folding transducer assembly
The transducer assembly consists primarily of a transducer and a horn.
Soundproof box
It can effectively reduce the noise generated during work and keep the laboratory quiet.
The promotion of ultrasonic cell disruption instrument in China has entered a mature stage, and the application is still not popular enough! The instrument (equipment) has a wide range of applications, which is unmatched by other instruments and equipment. For this reason, the market potential of the instrument (equipment) is very large, so the number of manufacturers is increasing, which also causes the relative confusion of the ultrasonic cleaning industry and the market. It can be used in eight words to describe the "mixed fish and dragons, mixed"! Classify ultrasonic cell disrupters.
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