This reagent is intended for research use only. The caspase-3 kit is a solid-phase sandwich enzyme-linked immunosorbent assay (ELISA) designed to quantify caspase-3 levels in biological samples. The procedure involves adding both standard solutions with known caspase-3 concentrations and unknown samples into microplate wells pre-coated with capture antibodies. Following an initial incubation, biotinylated detection antibodies are introduced, allowing them to bind to the captured caspase-3. After thorough washing to remove unbound components, avidin-conjugated horseradish peroxidase (HRP) is added. This step enables the formation of a stable antibody-enzyme complex. Another incubation period is followed by additional washing to eliminate any remaining unbound conjugate. Subsequently, substrates A and B are added simultaneously, triggering a colorimetric reaction. The intensity of the resulting color is directly proportional to the amount of caspase-3 present in the sample, allowing for accurate quantification through comparison with the standard curve. This method provides a reliable and sensitive approach for detecting caspase-3 activity in various experimental settings.
Napkin Holders,Napkin Holder,Utensil Drainer,Metal Napkin Holder,Kitchen Napkin Holder,Paper Napkin Holder
Jiangmen Jianghai Jianshang Houseware Co.,LTD. , https://www.jm-jianshang.com