1. The kit has been in transit for too long and the temperature is too high. Solution: Shorten the transportation time as much as possible, and put ice cubes to cool down in summer.
2. The kit is not fully balanced. Solution: Remove the reagent kit from the refrigerator at 2 ～ 8 ℃, open the lid, and equilibrate at room temperature for at least 20 minutes to ensure that all reagents have been equilibrated to room temperature (about 25 ℃).
3. The temperature of the incubator is less than 37 ℃. Solution: Pay attention to the temperature of the incubator. After placing the reaction plate, try to reduce the number of openings as much as possible to avoid affecting the constant temperature. Special attention should be paid to non-waterproof incubators.
4. Insulation time is insufficient. Solution: Correct the fixed clock for accurate timing.
5. The impact force during washing is too large, the soaking time is too long, and the number of washing times increases. Solution: Keep the washing time according to the instructions and remember the washing times accurately.
6. Insufficient pipette volume, too much water on the inner wall of the nozzle or the inner wall is not clean. Solution: calibrate the pipette, the nozzle should be matched, the nozzle should be tightly packed, and the inner wall of the nozzle should be cleaned, preferably one-time use.
7. The quality of distilled water is problematic. Solution: Use fresh qualified distilled water.
8. Insufficient substrate time. Solution: Accurate timing.
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