July is about to begin. Our company has launched a new product for ELISA kits in this issue. Our technical experts have analyzed their reference products (positive and negative). Let's take a look at it.
The positive control and the negative control are the control products for verifying the validity of the test, and also serve as a control for the judgment result. Therefore, the basic composition of the reference substance, especially the positive control substance, should be as consistent as possible with the composition of the test sample. For the determination of human serum as a specimen, the reference substance is preferably human serum, because normal human blood
Different degrees of background can be produced in various ELISA modes. Because a large number of normal human blood is more difficult to obtain, the reference materials in foreign kits mostly use recalcified human plasma as raw material, that is, calcium ions are added to the plasma to solidify the fiber protein, and the liquid obtained after removing the clot is The composition is similar to serum. The negative control must be tested first to determine that it does not contain the substance to be tested. For example, HBsAg should not contain HBsAg in the negative control, and anti-HBs are also negative. The positive control substance is mostly based on a buffer containing a protein protective agent, and a certain amount of the substance to be tested is added, and the amount is preferably indicated in the reagent specification. The amount to be added should be commensurate with the sensitivity of the reagent. The absorbance obtained in the assay is compared with the absorbance of the specimen to have a rough estimate of the amount of the test substance in the specimen. The sensitivity of the ELISA kit for detecting HBsAg abroad is about 0.5 ng/ml, and the content of the positive control is about 10 ng/ml. Antibiotics and preservatives are generally added to the control to facilitate storage.
Reference standard
Quantitatively determined ELISA kits should contain reference standards for the preparation of standard curves and should include 4-5 concentrations covering the detectable range, typically in buffers containing protein protectants and preservatives.
Goldstone Gemstone meaning
- Red goldstone is associated with vitality and energy.
- Blue Goldstone is associated with learning and communication.
- Green Goldstone is associated with growth and abundance.
Goldstone is a man made gemstone that has been around since the European Rennaissance.
Early monks working at a glass factory near Venice, Italy were practicing alchemy when molten copper accidentally tipped into a molten glass container.
This crystallized into thousands of tiny crystals and formed a melt with golden light sparkles. This is how the first goldstone came about. Though it is not technically a gemstone, it is often referred to as one.
Goldstone is an energy stone.
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