IncuCyte Zoom, the second generation of long-term dynamic live cell imaging and data analysis system of American Essen Company, is a set of long-term real-time dynamic, non-harmful live cell imaging analysis platform. The instrument is placed in an incubator, a standard culture container is placed in the middle, and a photograph is taken through the micro imaging device below. In addition to phase difference images, red fluorescence images and green fluorescence images, users can also obtain image-based graphs generated by the system software based on saturation and count analysis to show changes and trends in cells. For example, the saturation vs time chart showing cell proliferation, the relative wound density vs time chart showing the Wound Healing experiment, the blood vessel length vs time chart showing neovascularization, and the neurite length vs time chart showing nerve cell growth.
IncuCyte Zoom is divided into HD and 2CLR, HD can only do phase contrast imaging, and 2CLR can do phase contrast and red / green fluorescence imaging, and HD can be upgraded to 2CLR. The kit includes: nerve tracking kit, cell migration / invasion kit and angiogenesis kit.
Compared with the first-generation imager IncuCyte FLR, IncuCyte Zoom is a new design that adds a lot of key performance, including support for multi-channel fluorescence imaging, support for multiple objective lens selection, increased morphological processing capabilities and higher speed. The newly added features are: 1) IncuCyte Zoom can support high-definition (HD) phase contrast, yellow-green fluorescence and red fluorescence automatic imaging functions, and the image processing software supports the integrated processing of three-channel imaging; 2) the user can be in 1 minute Replace 4 ×, 10 × and 20 × objective lenses without the help of professionals; 3) Because of having 12 full-core processors and new software and hardware, the imaging speed is twice that of the first generation FLR; 4 ) A new camera design is adopted to make it more suitable for long-term observation; 5) A new database is designed to make it easier for users to find and retrieve experimental data; 6) Increased data storage capacity, equipped with 9TB storage Space, and support for external data storage systems; 7) Increased air flow rate, improved heat dissipation performance, and increased scan time; 8) Increased system monitoring, integrating humidity sensors and accelerometers to monitor unacceptable vibration, fan speed, and Internal temperature; 9) Users can use the LCD control panel located at the front of the control machine to easily detect and run basic status checks and instrument operations.
The advantages of IncuCyte: 1) The cultured cells are directly monitored with a high-definition phase contrast microscope or a fluorescence microscope, which is non-destructive monitoring. The cells can be monitored without staining and the image effect is good; 2) The cells do not need to leave the incubator during the monitoring , Don't worry about the influence of the change of culture conditions on the cells; 3) True long-term dynamic live cell imaging, up to days or months, high-resolution phase contrast imaging can eliminate the meniscus defects of microplates, and there is no The halo problem of traditional microscopic systems can monitor cell morphology in 384 microwell plates; 4) Image processing software automatically quantifies cell proliferation based on saturation and count analysis; 5) Automatically output cell growth curves and cell growth videos; 6 ) Real-time monitoring can change the traditional "end point method" experimental method, data is rich, more accurate and more flexible; 7) high throughput, can simultaneously monitor 6 standard specifications of cell culture plates / dishes / bottles, cells at 384 micro In-well plate experiments can reduce the consumption of precious medicines; 8) support more than 200 kinds of standard culture vessels, no special culture vessels are needed, saving later experiment costs; 9) remote monitoring, Perform data reading and analysis without repeated access to the cell room, avoiding the potential pollution hazards and the trouble of manual entry and exit observation.
Applications of IncuCyte Zoom include:
1) Monitor cell proliferation: Non-invasive and quantitative analysis of cell proliferation through real-time dynamic imaging;
2) Cell culture quality control / cell culture optimization: through real-time dynamic imaging, a dynamic growth curve based on cell morphology and saturation can be generated;
3) Monitoring cytotoxicity: mixing dye and cells and then reading, the operator can leave, is based on dye experiments;
4) Monitor apoptosis: mix apoptosis reagents and cells and then read, the operator can leave, it is a dye-based experiment;
5) Monitoring cell migration / cell invasion: supporting a highly consistent 96-hole wound scratch tool, real-time dynamic monitoring of wound healing process, quantitative indicators of cell movement can be obtained;
6) Monitoring of angiogenesis: using a validated angiogenesis kit with quantitative indicators for dynamic analysis of angiogenesis;
7) Monitoring report genes: cells do not need to be lysed, and real-time dynamic data can be obtained;
8) Nerve growth tracking: quantitative analysis of neuronal cell neurite growth;
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