The solid phase extraction extraction method is divided into three steps:
(a) bringing together relevant information;
(b) establishing a preliminary solid phase extraction method;
(c) optimizing the parameters in the method;
The automatic solid phase extraction instrument AutoSPE control software is a user-friendly and graphical Chinese operation software. The user's needs are fully considered from the system configuration and method establishment to the establishment of special instructions. The software's built-in SPE instructions include activation, loading, rinsing, drying, elution, and liquid processing instructions commonly used in laboratories to make method creation and optimization easier.
Common problems in solid phase extraction:
(1) Flow rate problem: factors such as vacuum, pressure, gravity, etc. all affect the flow rate of liquid through the SPE column. A stable flow rate is important for extraction efficiency, especially for sample column and elution steps. Too fast a flow rate will reduce the recovery of the target compound, especially solid phase extraction using ion exchange as a mechanism. Slow flow rates increase analysis time and reduce work efficiency.
The use of six high-precision ceramic metering pumps, positive pressure loading, to ensure accurate flow rate at a steady flow rate according to the specified sample volume, to ensure high accuracy and repeatability of the analysis results.
(2) Recovery rate problem: Generally speaking, when the extraction recovery result is not satisfactory, it should be carefully and systematically excluded to find out the cause, and then the extraction method is improved. Establish a stable and reliable method, the flow rate can directly affect the final result, therefore, it is important to control the flow rate.
(3) Pollution problem: The pollution generated by the column depends to a large extent on the concentration of the target compound and the means of detection used. Manufacturers with quality control can usually control contaminants in the range of PPb levels, below which contaminants may be present. If these contaminants interfere with the detection of the target compound, the column should be pre-cleaned.
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